Selected article for: "pH Tris HCl buffer and Tris HCl buffer"
Author: Wanchao Yin; Chunyou Mao; Xiaodong Luan; Dan-Dan Shen; Qingya Shen; Haixia Su; Xiaoxi Wang; Fulai Zhou; Wenfeng Zhao; Minqi Gao; Shenghai Chang; Yuan-Chao Xie; Guanghui Tian; He-Wei Jiang; Sheng-Ce Tao; Jingshan Shen; Yi Jiang; Hualiang Jiang; Yechun Xu; Shuyang Zhang; Yan Zhang; H. Eric Xu
Title: Structural Basis for the Inhibition of the RNA-Dependent RNA Polymerase from SARS-CoV-2 by Remdesivir
  • Document date: 2020_4_9
    • ID: 7v7pzclb_37
    Snippet: A short RNA oligonucleotide of sequence 5'-FAM-GCUAUGUGAGAUUAAGAAUU-3' was used as the primer strand and a longer RNA oligonucleotide of sequence 5'-UUUUUUUUUUAAUUCUUAAUCUCACAUAGC -3' was used as template strand. To anneal the RNA duplex, both oligonucleotides were mixed at equimolar ratios in annealing buffer (10 mM Tris-HCl pH 8.0, 25 mM NaCl and 2.5 mM EDTA), denatured by heating to 94℃ for 5 min and allowed to slowly cool to room temperatur.....
    Document: A short RNA oligonucleotide of sequence 5'-FAM-GCUAUGUGAGAUUAAGAAUU-3' was used as the primer strand and a longer RNA oligonucleotide of sequence 5'-UUUUUUUUUUAAUUCUUAAUCUCACAUAGC -3' was used as template strand. To anneal the RNA duplex, both oligonucleotides were mixed at equimolar ratios in annealing buffer (10 mM Tris-HCl pH 8.0, 25 mM NaCl and 2.5 mM EDTA), denatured by heating to 94℃ for 5 min and allowed to slowly cool to room temperature.

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