Author: Hecht, Leonie-Sophie; Jurado-Jimenez, Angeles; Hess, Markus; Halas, Hussein El; Bochenek, Gregor; Mohammed, Hala; Alzahrani, Fahd; Asiri, Mohammed O; Hasan, Rami; Alamri, Aref; Alotaibi, Sultan
Title: Verification and diagnostic evaluation of the RealStar(®) Middle East respiratory syndrome coronavirus (N gene) reverse transcription-PCR kit 1.0 Cord-id: qj45jefm Document date: 2019_7_2
ID: qj45jefm
Snippet: AIM: We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene. MATERIAL & METHODS: 33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV orf1a and upE, and were tested using the MERS-CoV N gene, as a confirmatory assay. This diagnostic procedure follows a two-step diagnostics scheme, recommended by t
Document: AIM: We report the diagnostic evaluation of a confirmatory reverse transcription-PCR (RT-PCR) kit targeting the Middle East respiratory syndrome coronavirus (MERS-CoV) N gene. MATERIAL & METHODS: 33 patient samples from two collections sites in Riyadh, Saudi Arabia, which were pre-characterized via real-time RT-PCR targeting MERS-CoV orf1a and upE, and were tested using the MERS-CoV N gene, as a confirmatory assay. This diagnostic procedure follows a two-step diagnostics scheme, recommended by the WHO. RESULTS: 18/33 samples tested positive, 11/33 tested negative for MERS-CoV RNA and 2/33 showed uncertain results. CONCLUSION: The results suggest, that the RealStar(®) MERS-CoV (N gene) RT-PCR kit 1.0 can be considered a suitable and reliable confirmatory assay in combination with the RealStar MERS-CoV RT-PCR kit 1.0 according to the diagnostic scheme recommended by WHO.
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