Selected article for: "final concentration and RT reaction"
Author: Arunkumar Arumugam; Season S Wong
Title: The Potential Use of Unprocessed Sample for RT-qPCR Detection of COVID-19 without an RNA Extraction Step
  • Document date: 2020_4_8
    • ID: ka3n9pft_22
    Snippet: TaqPath 1-step multiplex master mix was used for the RT-qPCR reaction with specific primers and probes tagged with FAM. The final concentration of primers and probe sets for influenza A, B and RSV in the reaction is 250 nM in a 20 µL reaction. 6, 7 For the detection of SARS-CoV-2 target genes, N1, N2 and N3, the final concentration of primers are 500 nM and probes are 125 nM as per the CDC protocol. 8 Table 1 The copyright holder for this prepri.....
    Document: TaqPath 1-step multiplex master mix was used for the RT-qPCR reaction with specific primers and probes tagged with FAM. The final concentration of primers and probe sets for influenza A, B and RSV in the reaction is 250 nM in a 20 µL reaction. 6, 7 For the detection of SARS-CoV-2 target genes, N1, N2 and N3, the final concentration of primers are 500 nM and probes are 125 nM as per the CDC protocol. 8 Table 1 The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.06.028811 doi: bioRxiv preprint

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