Author: Arunkumar Arumugam; Season S Wong
Title: The Potential Use of Unprocessed Sample for RT-qPCR Detection of COVID-19 without an RNA Extraction Step Document date: 2020_4_8
ID: ka3n9pft_22
Snippet: TaqPath 1-step multiplex master mix was used for the RT-qPCR reaction with specific primers and probes tagged with FAM. The final concentration of primers and probe sets for influenza A, B and RSV in the reaction is 250 nM in a 20 µL reaction. 6, 7 For the detection of SARS-CoV-2 target genes, N1, N2 and N3, the final concentration of primers are 500 nM and probes are 125 nM as per the CDC protocol. 8 Table 1 The copyright holder for this prepri.....
Document: TaqPath 1-step multiplex master mix was used for the RT-qPCR reaction with specific primers and probes tagged with FAM. The final concentration of primers and probe sets for influenza A, B and RSV in the reaction is 250 nM in a 20 µL reaction. 6, 7 For the detection of SARS-CoV-2 target genes, N1, N2 and N3, the final concentration of primers are 500 nM and probes are 125 nM as per the CDC protocol. 8 Table 1 The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.06.028811 doi: bioRxiv preprint
Search related documents:
Co phrase search for related documents- CDC protocol and master mix: 1
- CDC protocol and probe set: 1
- CDC protocol and probe set primer: 1
- CDC protocol and RT qPCR reaction: 1, 2
- CDC protocol and target gene: 1
- final concentration and master mix: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- final concentration and primer final concentration: 1, 2, 3, 4, 5, 6, 7
- final concentration and probe set: 1, 2, 3
- final concentration and probe set primer: 1, 2, 3
- final concentration and probe specific primer: 1, 2, 3
- final concentration and specific primer: 1, 2, 3, 4
- final concentration and target gene: 1
- master mix and multiplex master mix: 1, 2
- master mix and primer final concentration: 1, 2, 3
- master mix and RT qPCR reaction: 1, 2, 3, 4
- master mix and SARS target gene: 1
- master mix and specific primer: 1, 2, 3, 4, 5, 6, 7
- master mix and target gene: 1, 2, 3, 4, 5
- primer final concentration and specific primer: 1
Co phrase search for related documents, hyperlinks ordered by date