Author: Joseph C. Ward; Lidia Lasecka-Dykes; Chris Neil; Oluwapelumi Adeyemi; Sarah Gold; Niall McLean; Caroline Wright; Morgan R. Herod; David Kealy; Emma Warner; Donald P. King; Tobias J. Tuthill; David J. Rowlands; Nicola J. Stonehouse
Title: The RNA pseudoknots in foot-and-mouth disease virus are dispensable for genome replication but essential for the production of infectious virus Document date: 2020_1_11
ID: ahrrphm8_13
Snippet: Transcription reactions to produce large amounts of RNA for SHAPE analysis were performed 145 with purified linear DNA as described above, and 1 μg of linearised DNA was then used in a 146 HiScribe T7 synthesis kit (NEB), before DNase treatment and purification using a PureLink FastQ files were quality checked using FastQC with poor quality reads filtered using the 225 Sickle algorithm. Host cell reads were removed using FastQ Screen algorithm a.....
Document: Transcription reactions to produce large amounts of RNA for SHAPE analysis were performed 145 with purified linear DNA as described above, and 1 μg of linearised DNA was then used in a 146 HiScribe T7 synthesis kit (NEB), before DNase treatment and purification using a PureLink FastQ files were quality checked using FastQC with poor quality reads filtered using the 225 Sickle algorithm. Host cell reads were removed using FastQ Screen algorithm and FMDV 226 reads assembled de novo into contigs using IDBA-UD (35). Contigs that matched the FMDV 227 library (identified using Basic Local ALighnment Search Tool (BLAST)) were assembled 228 author/funder. All rights reserved. No reuse allowed without permission.
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