Author: Xufang Deng; Yafang Chen; Anna M. Mielech; Matthew Hackbart; Kristina R. Kesely; Robert C. Mettelman; Amornrat O’Brien; Mackenzie E. Chapman; Andrew D. Mesecar; Susan C. Baker
Title: Structure-Guided Mutagenesis Alters Deubiquitinating Activity and Attenuates Pathogenesis of a Murine Coronavirus Document date: 2019_9_25
ID: l3qp0n9f_13
Snippet: The structure-guided approach used to generate the DUBmut virus allowed for substitution. The deISGylating ability of the enzyme is also not affected. In contrast, the mutant 237 enzyme's deubiquitinating activity is significantly reduced relative to wild-type, which is most 238 likely due to a lowered binding affinity for ubiquitin as the enzyme could not be saturated with 239 Ub-AMC as a substrate. 240 All rights reserved. No reuse allowed with.....
Document: The structure-guided approach used to generate the DUBmut virus allowed for substitution. The deISGylating ability of the enzyme is also not affected. In contrast, the mutant 237 enzyme's deubiquitinating activity is significantly reduced relative to wild-type, which is most 238 likely due to a lowered binding affinity for ubiquitin as the enzyme could not be saturated with 239 Ub-AMC as a substrate. 240 All rights reserved. No reuse allowed without permission.
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