Author: Wioletta Rut; Katarzyna Groborz; Linlin Zhang; Xinyuanyuan Sun; Mikolaj Zmudzinski; Rolf Hilgenfeld; Marcin Drag
Title: Substrate specificity profiling of SARS-CoV-2 Mpro protease provides basis for anti-COVID-19 drug design Document date: 2020_3_8
ID: e8qubwha_13
Snippet: Fmoc-Gln(Trt)-OH (24.05 mmol, 14.69 g) with 2.5 eq HATU (24.05 mmol, 9.15 g), 2.5 eq collidine (24.05 mmol, 3.18 mL) in DMF were activated for 2 min and added to filter cannula with 1 eq (9.62 mmol) H 2 N-ACC-resin and the reaction was carried out for 24 h. Next, the resin was washed four times with DMF and the same reaction was performed again using 1.5 eq of above reagents. After four DMF washes, the Fmoc protecting group was removed using 20% .....
Document: Fmoc-Gln(Trt)-OH (24.05 mmol, 14.69 g) with 2.5 eq HATU (24.05 mmol, 9.15 g), 2.5 eq collidine (24.05 mmol, 3.18 mL) in DMF were activated for 2 min and added to filter cannula with 1 eq (9.62 mmol) H 2 N-ACC-resin and the reaction was carried out for 24 h. Next, the resin was washed four times with DMF and the same reaction was performed again using 1.5 eq of above reagents. After four DMF washes, the Fmoc protecting group was removed using 20% piperidine in DMF (5, 5, The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.07.981928 doi: bioRxiv preprint MultiChem 48-wells synthesis apparatus (FlexChem from SciGene, Sunnyvale, CA, USA).
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