Author: Linlin Zhang; Daizong Lin; Yuri Kusov; Yong Nian; Qingjun Ma; Jiang Wang; Albrecht von Brunn; Pieter Leyssen; Kristina Lanko; Johan Neyts; Adriaan de Wilde; Eric J. Snijder; Hong Liu; Rolf Hilgenfeld
Title: Alpha-ketoamides as broad-spectrum inhibitors of coronavirus and enterovirus replication Document date: 2020_2_10
ID: 7n8p9okf_55
Snippet: Crystals of HCoV-NL63 M pro with 11n or 11f were generated by using the soaking method. Several free-enzyme crystals were soaked in cryo-protectant buffer containing 0.1 M lithium sulfate monohydrate, 0.1 M sodium citrate tribasic dihydrate, 25% PEG 1,000, 15% glycerol, 5 mM 11n (or 11f), pH 6.0. Subsequently, the soaked crystals were flash-cooled in liquid nitrogen......
Document: Crystals of HCoV-NL63 M pro with 11n or 11f were generated by using the soaking method. Several free-enzyme crystals were soaked in cryo-protectant buffer containing 0.1 M lithium sulfate monohydrate, 0.1 M sodium citrate tribasic dihydrate, 25% PEG 1,000, 15% glycerol, 5 mM 11n (or 11f), pH 6.0. Subsequently, the soaked crystals were flash-cooled in liquid nitrogen.
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