The role of post-Golgi transport pathways and sorting motifs in the plasmodesmal targeting of the movement protein (MP) of Ourmia melon virus (OuMV)

Natali Ozber; Paolo Margaria; Charles T. Anderson; Massimo Turina; Cristina Rosa

GFP:MP_D/G, able to support movement, exhibited a similar localization pattern compared to GFP:MPwt and the intensity profile across the PD strongly coaligned with . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/724716 doi: bioRxiv preprint that of aniline blue (Fig. 5B ). Although peripheral punctate structures marked by GFP:MP_D and GFP:MP_LI/GG did not predominantly localize to callose deposits ( Fig. 5C -D), GFP:MP_LI/GG labeled structures were in close proximity of PD (Fig. 5D ). The quantification of particles labeled by GFP:MPwt or GFP:MP mutants and aniline blue revealed that some of GFP:MP_D and GFP:MP_LI/GG co-localize with callose deposits, but the number of co-localized particles was much lower than that of GFP:MPwt and GFP:MP_D/G (Fig. 5E ). The statistical analysis of co-localized particles revealed significant differences between GFP:MPwt or GFP:MP_D/G and movement deficient MP mutants, GFP:MP_D and GFP:MP_LI/GG (Fig. 5F ). In contrast, no significant difference between GFP:MPwt and GFP:MP_D/G was observed (Fig. 5F ).