The role of post-Golgi transport pathways and sorting motifs in the plasmodesmal targeting of the movement protein (MP) of Ourmia melon virus (OuMV)

Natali Ozber; Paolo Margaria; Charles T. Anderson; Massimo Turina; Cristina Rosa

. CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/724716 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/724716 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/724716 doi: bioRxiv preprint mutants, (B) GFP:MP_D/G, (C) GFP:MP_D, and (D) GFP:MP_LI/GG (magenta) with RdRp and CP. Aniline blue was used to mark callose deposits (cyan). Selected regions (white rectangles) show the intensity profile of the diagonal line generated from images that were adjusted to a lower intensity by ImageJ. Images represent single optical planes. Scale bar, 20μm, 10 μm (magnified region). (E) The quantification of callose deposits (blue), punctate structures labeled by OuMV GFP:MPwt or GFP:MP mutants (magenta), and co-localized particles (gray) per area of view. The particles were counted using the ComDet v.0.4.1 plugin in ImageJ. At least 6 areas of view were examined (n≥6) and the total number of counted particles for each sample is more than 700. Error bars indicate standard error of the mean. (F) The statistical analysis of colocalized particles in (E). Bar graph represents PD-localized OuMV GFP:MPwt or GFP:MP mutants per area of view (mean±SE, n≥6). ***, P < 0.001 by ANOVA, followed by Tukey's HSD.