Selected article for: "assay buffer and final concentration"
Author: Wioletta Rut; Katarzyna Groborz; Linlin Zhang; Xinyuanyuan Sun; Mikolaj Zmudzinski; Rolf Hilgenfeld; Marcin Drag
Title: Substrate specificity profiling of SARS-CoV-2 Mpro protease provides basis for anti-COVID-19 drug design
  • Document date: 2020_3_8
    • ID: e8qubwha_16
    Snippet: Hybrid combinatorial substrate library screening was performed using a spectrofluorometer (Molecular Devices Spectramax Gemini XPS) in 384-well plates (Corning). The assay conditions were as follows: 1 µL of substrate and 49 µL of enzyme, which was incubated at 37°C for 10 min in assay buffer (20 mM Tris, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.3). The final substrate concentration was 100 µM and the final enzyme concentration was 1 µM SARS-C.....
    Document: Hybrid combinatorial substrate library screening was performed using a spectrofluorometer (Molecular Devices Spectramax Gemini XPS) in 384-well plates (Corning). The assay conditions were as follows: 1 µL of substrate and 49 µL of enzyme, which was incubated at 37°C for 10 min in assay buffer (20 mM Tris, 150 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.3). The final substrate concentration was 100 µM and the final enzyme concentration was 1 µM SARS-CoV and 0.6 µM SARS-CoV-2 M pro , respectively.

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