Selected article for: "Firefly luciferase and luciferase activity"
Author: Linlin Zhang; Daizong Lin; Yuri Kusov; Yong Nian; Qingjun Ma; Jiang Wang; Albrecht von Brunn; Pieter Leyssen; Kristina Lanko; Johan Neyts; Adriaan de Wilde; Eric J. Snijder; Hong Liu; Rolf Hilgenfeld
Title: Alpha-ketoamides as broad-spectrum inhibitors of coronavirus and enterovirus replication
  • Document date: 2020_2_10
    • ID: 7n8p9okf_9
    Snippet: To enable the rapid and biosafe screening of antivirals against corona-and enteroviruses, a non-infectious, but replication-competent SARS-CoV replicon was used 33 along with subgenomic replicons of CVB3 34 and EV-A71 (kind gift of B. Zhang, Wuhan, China). The easily detectable reporter activity (firefly or Renilla luciferase) of these replicons has previously been shown to reflect viral RNA synthesis. [33] [34] [35] In-vitro RNA transcripts of t.....
    Document: To enable the rapid and biosafe screening of antivirals against corona-and enteroviruses, a non-infectious, but replication-competent SARS-CoV replicon was used 33 along with subgenomic replicons of CVB3 34 and EV-A71 (kind gift of B. Zhang, Wuhan, China). The easily detectable reporter activity (firefly or Renilla luciferase) of these replicons has previously been shown to reflect viral RNA synthesis. [33] [34] [35] In-vitro RNA transcripts of the enteroviral replicons were also used for transfection. For the SARS-CoV replicon containing the CMV promoter, only the plasmid DNA was used for transfection.

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