The guanine nucleotide exchange factor GBF1 participates in rotavirus replication

José L. Martínez; Francesca Arnoldi; Elisabeth M. Schraner; Catherine Eichwald; Daniela Silva-Ayala; Eunjoo Lee; Elizabeth Sztul; Óscar R. Burrone; Susana López; Carlos F. Arias

The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/619924 doi: bioRxiv preprint 8 Similarly, treatment with GCA diminished viral progeny about 50-fold ( Fig 1B) . Cell 149 treatment with the inhibitors did not alter cell viability at any of the concentrations tested, as 150 determined by an LDH assay (data not shown). Moreover, the reduction of viral yield was 151 independent of the rotavirus strain tested, since BFA also significantly inhibited the 152 replication of SA11 (simian origin), UK (bovine origin), 69M (human origin), and YM 153 (porcine origin) ( Fig 1C) . However, the extent of inhibition differed among the strains; while 154 the replication of SA11 was reduced to a level similar to RRV (about 100-fold), that of 155 rotavirus strains UK, 69M, and YM was reduced by about 10-fold.