Author: Natali Ozber; Paolo Margaria; Charles T. Anderson; Massimo Turina; Cristina Rosa
Title: The role of post-Golgi transport pathways and sorting motifs in the plasmodesmal targeting of the movement protein (MP) of Ourmia melon virus (OuMV) Document date: 2019_8_11
ID: bvcahbbi_25
Snippet: OuMV MP by a pull-down assay. His-CesA1 has been previously reported as AP2M-MHD interacting protein (Bashline et al., 2013) and was used a positive control in the pull-down assay. Even though the positive control clearly bound AP2M-MHD, we were . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/724716 doi: bioRxi.....
Document: OuMV MP by a pull-down assay. His-CesA1 has been previously reported as AP2M-MHD interacting protein (Bashline et al., 2013) and was used a positive control in the pull-down assay. Even though the positive control clearly bound AP2M-MHD, we were . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/724716 doi: bioRxiv preprint unable to detect binding of AP2M with MP in vitro (Fig. 7) . As opposed to CaMV MP, OuMV MP does not directly interact with AP2M under tested conditions.
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