Author: Bruni, Margherita; Cecatiello, Valentina; Diaz-Basabe, Angelica; Lattanzi, Georgia; Mileti, Erika; Monzani, Silvia; Pirovano, Laura; Rizzelli, Francesca; Visintin, Clara; Bonizzi, Giuseppina; Giani, Marco; Lavitrano, Marialuisa; Faravelli, Silvia; Forneris, Federico; Caprioli, Flavio; Pelicci, Pier Giuseppe; Natoli, Gioacchino; Pasqualato, Sebastiano; Mapelli, Marina; Facciotti, Federica
Title: Persistence of Anti-SARS-CoV-2 Antibodies in Non-Hospitalized COVID-19 Convalescent Health Care Workers Cord-id: ybdkp398 Document date: 2020_10_1
ID: ybdkp398
Snippet: Although antibody response to SARS-CoV-2 can be detected early during the infection, several outstanding questions remain to be addressed regarding the magnitude and persistence of antibody titer against different viral proteins and their correlation with the strength of the immune response. An ELISA assay has been developed by expressing and purifying the recombinant SARS-CoV-2 Spike Receptor Binding Domain (RBD), Soluble Ectodomain (Spike), and full length Nucleocapsid protein (N). Sera from h
Document: Although antibody response to SARS-CoV-2 can be detected early during the infection, several outstanding questions remain to be addressed regarding the magnitude and persistence of antibody titer against different viral proteins and their correlation with the strength of the immune response. An ELISA assay has been developed by expressing and purifying the recombinant SARS-CoV-2 Spike Receptor Binding Domain (RBD), Soluble Ectodomain (Spike), and full length Nucleocapsid protein (N). Sera from healthcare workers affected by non-severe COVID-19 were longitudinally collected over four weeks, and compared to sera from patients hospitalized in Intensive Care Units (ICU) and SARS-CoV-2-negative subjects for the presence of IgM, IgG and IgA antibodies as well as soluble pro-inflammatory mediators in the sera. Non-hospitalized subjects showed lower antibody titers and blood pro-inflammatory cytokine profiles as compared to patients in Intensive Care Units (ICU), irrespective of the antibodies tested. Noteworthy, in non-severe COVID-19 infections, antibody titers against RBD and Spike, but not against the N protein, as well as pro-inflammatory cytokines decreased within a month after viral clearance. Thus, rapid decline in antibody titers and in pro-inflammatory cytokines may be a common feature of non-severe SARS-CoV-2 infection, suggesting that antibody-mediated protection against re-infection with SARS-CoV-2 is of short duration. These results suggest caution in using serological testing to estimate the prevalence of SARS-CoV-2 infection in the general population.
Search related documents:
Co phrase search for related documents- absolute frequency and longitudinal analysis: 1
- active recruitment and acute phase: 1, 2, 3, 4
- active recruitment and acute respiratory: 1, 2, 3
- acute phase and adaptive immune cell: 1, 2
- acute phase and longitudinal analysis: 1, 2, 3, 4
- acute phase and longitudinal study: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- acute phase and longitudinal variation: 1
- acute phase and longitudinally follow: 1
- acute respiratory and adaptive immune cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- acute respiratory and adaptive immune cell innate: 1, 2, 3, 4, 5, 6, 7
- acute respiratory and longitudinal analysis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- acute respiratory and longitudinal study: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- acute respiratory and longitudinal variation: 1, 2
- acute respiratory and low antibody titer: 1, 2
- acute respiratory distress syndrome ards and adaptive immune cell: 1, 2
- acute respiratory distress syndrome ards and adaptive immune cell innate: 1, 2
- acute respiratory distress syndrome ards and longitudinal analysis: 1, 2
- acute respiratory distress syndrome ards and longitudinal study: 1, 2, 3, 4, 5
- address remain and longitudinal study: 1
Co phrase search for related documents, hyperlinks ordered by date