Monoclonal Antibody HlyIIC‑15 to C-End Domain HlyII B. cereus Interacts with the Trombin Recognition Site

Zamyatina, A. V.; Rudenko, N. V.; Karatovskaya, A. P.; Shepelyakovskaya, A. O.; Siunov, A. V.; Andreeva-Kovalevskaya, Zh. I.; Nagel, A. S.; Salyamov, V. I.; Kolesnikov, A. S.; Brovko, F. A.; Solonin, A. S.

Among the panel of monoclonal antibodies to the recombinant protein HlyIICTD Bacillus cereus an antibody was found capable of forming an immune complex with a thrombin recognition region, the amino acid sequence of which is located inside the recombinant HlyIICTD. Localization of the epitope was carried out using peptide phage display methods, as well as enzyme immunoassay and immunoblotting for interaction with recombinant proteins, either containing or not containing individual components HlyIICTD. The identified epitope is located in the region of the thrombin site and retains the ability to interact with the antibody after the proteolyotic attack of the protein by thrombin.