Intracellular host cell membrane remodelling induced by SARS‐CoV‐2 infection in vitro

Caldas, Lucio Ayres; Carneiro, Fabiana Avila; Monteiro, Fabio Luis; Augusto, Ingrid; Higa, Luiza Mendonça; Miranda, Kildare; Tanuri, Amilcar; de Souza, Wanderley

BACKGROUND INFORMATION: Severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2) infection induces an alteration in the endomembrane system of the mammalian cells. In this study, we used transmission electron microscopy and electron tomography to investigate the main structural alterations in the cytoplasm of Vero cells infected with a SARS‐CoV‐2 isolate from São Paulo state (Brazil). RESULTS: Different membranous structures derived from the zippered endoplasmic reticulum were observed along with virus assembly through membrane budding. Also, we demonstrated the occurrence of annulate lamellae in the cytoplasm of infected cells and the presence of virus particles in the perinuclear space. CONCLUSIONS AND SIGNIFICANCE: This study contributes to a better understanding of the cell biology of SARS‐CoV‐2 and the mechanisms of the interaction of the virus with the host cell that promote morphological changes, recruitment of organelles and cell components, in a context of a virus‐induced membrane remodelling.