Author: Arunkumar Arumugam; Season S Wong
Title: The Potential Use of Unprocessed Sample for RT-qPCR Detection of COVID-19 without an RNA Extraction Step Document date: 2020_4_8
ID: ka3n9pft_12
Snippet: Our data using both high (Ct < 35) and low (Ct >35) target concentrations suggest that since RT-qPCR is highly sensitive, using raw samples or minimal sample preparation steps might not reduce the test sensitivity as most patients tend to have a higher viral load. 5 Also, the efficiency of extraction methods tends to drop significantly at very low target concentrations when processed through numerous washing steps. Therefore, nucleic acid loss du.....
Document: Our data using both high (Ct < 35) and low (Ct >35) target concentrations suggest that since RT-qPCR is highly sensitive, using raw samples or minimal sample preparation steps might not reduce the test sensitivity as most patients tend to have a higher viral load. 5 Also, the efficiency of extraction methods tends to drop significantly at very low target concentrations when processed through numerous washing steps. Therefore, nucleic acid loss during extraction steps may hurt the limit of detection. While further studies with patient specimens and a higher number of samples are needed to confirm our preliminary results, we report that the use of untreated samples can be a viable option during the COVID-19 pandemic.
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