Alpha Herpesvirus Egress and Spread from Neurons Uses Constitutive Secretory Mechanisms Independent of Neuronal Firing Activity

Anthony E. Ambrosini; Nikhil Deshmukh; Michael J. Berry; Lynn W. Enquist; Ian B. Hogue

238 To directly measure PRV egress from infected cells, we previously developed a 239 live-cell fluorescence microscopy assay of virus particle exocytosis [14, 15] . We 303 however, we show here that Rab11a does not appear to be associated with virus 304 particle exocytosis in neurons (Fig 5H) , consistent with the divergent roles of . CC-BY 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/729830 doi: bioRxiv preprint 305 Rab11 proteins in neurons versus non-neuronal cells [19] . Finally, Rab3a, which 306 mediates the trafficking and Ca 2+ -regulated exocytosis of synaptic vesicles in 307 neurons, also does not appear to be associated with virus particle exocytosis (Fig 308 5I) . Altogether, these results are consistent with PRV particles using the 309 constitutive secretory pathway, not Ca 2+ -regulated synaptic vesicle-like secretory 310 mechanisms, even in neurons. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/729830 doi: bioRxiv preprint 697 neurons. Data represent 41 exocytosis events. (I) Rab3a is not associated with 698 virus particle exocytosis in neurons. Data represent 64 exocytosis events.