Author: Lulin Zhou; Zubiao Niu; Xiaoyi Jiang; Zhengrong Zhang; You Zheng; Zhongyi Wang; Yichao Zhu; Lihua Gao; Xiaoning Wang; Qiang Sun
Title: Systemic analysis of tissue cells potentially vulnerable to SARS-CoV-2 infection by the protein-proofed single-cell RNA profiling of ACE2, TMPRSS2 and Furin proteases Document date: 2020_4_10
ID: 3btc31kj_11
Snippet: To avoid batch effects among samples and experiments in the heart and intestine datasets, we adopted the anchoring procedure in the Seurat R package to integrate the datasets as described previously [38] . In brief, the "anchor" correspondences between datasets were identified using the "Find Integration Anchors" function with default parameters in Seurat. And then, we used the "Integrate Data" function in Seurat to obtain the batchcorrected expr.....
Document: To avoid batch effects among samples and experiments in the heart and intestine datasets, we adopted the anchoring procedure in the Seurat R package to integrate the datasets as described previously [38] . In brief, the "anchor" correspondences between datasets were identified using the "Find Integration Anchors" function with default parameters in Seurat. And then, we used the "Integrate Data" function in Seurat to obtain the batchcorrected expression matrices. we also applied another integration approach called "Harmony" [39] to the kidney and testis datasets. The Harmony R package (version1.0, https://github.com/immunogenomics/Harmony) focuses on scalable integration of the scRNA-seq data for batch correction.
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