Protection from infection with severe acute respiratory syndrome coronavirus in a Chinese hamster model by equine neutralizing F(ab')2.

Luo, Deyan; Ni, Bing; Zhao, Guangyu; Jia, Zhengcai; Zhou, Lili; Pacal, Marek; Zhang, Liangyan; Zhang, Songle; Xing, Li; Lin, Zhihua; Wang, Li; Li, Jintao; Liang, Yunfei; Shi, Xinfu; Zhao, Tingting; Zou, Liyun; Wu, Yuzhang; Wang, Xiliang

To warrant potential clinical testing, the equine anti-severe acute respiratory syndrome coronavirus (SARS-CoV) F(ab')(2) requires evaluation in as many animal models as possible. In this study, we established a new animal model, the Chinese hamster, susceptible to SARS-CoV infection. SARS-CoV could propagate effectively and sustain high levels for 1 wk in animal lungs. All animals were protected from SARS-CoV infection in preventive settings. Further, when used therapeutically this antibody led to an approximately 4-log(10) decrease in viral burden in infected animal lungs. The pathological changes in lungs correlated closely with the dose of antibody administered. The excellent preventive and therapeutic roles of equine anti-SARS-CoV F(ab')(2) in several animal models, including the novel Chinese hamster model described in this study, have provided exciting data concerning its potential clinical study.