Author: Xiankun Zeng; Jun Liu; April Babka; Brian Kearney; Sheli Radoshitzky; Jens Kuhn
Title: Molecular Detection of SARS-CoV-2 in Formalin Fixed Paraffin Embedded Specimens Document date: 2020_4_22
ID: nzwe7hpg_24
Snippet: As worldwide infectious disease researchers are racing to understand the pathogenesis of and to 128 develop and evaluate MCMs against COVID-19 to contain the ongoing pandemic, assays that 129 determine SARS-CoV-2 distribution in tissues and specific cellular targets of infection are 130 urgently needed. Here we evaluated commercial reagents and assays to detect SARS-CoV-2 131 antigens or RNA in FFPE specimens. We identified one rabbit polyclonal .....
Document: As worldwide infectious disease researchers are racing to understand the pathogenesis of and to 128 develop and evaluate MCMs against COVID-19 to contain the ongoing pandemic, assays that 129 determine SARS-CoV-2 distribution in tissues and specific cellular targets of infection are 130 urgently needed. Here we evaluated commercial reagents and assays to detect SARS-CoV-2 131 antigens or RNA in FFPE specimens. We identified one rabbit polyclonal antibody and one 132 mouse monoclonal antibody that reacts with SARS-CoV-2 S and N, respectively, and 133 demonstrated that these two antibodies can be used to detect SARS-CoV-2 by IHC and IFA in 134 FFPE specimens. Additionally, we characterized two RNAscope ISH assays that can be used to 135 detect SARS-CoV-2 positive-and negative-sense RNAs in FFPE specimens. Furthermore, we 136 developed a dual staining assay using IHC and ISH to detect SARS-CoV-2 S and RNA in the 137 same FFPE section. These reagents and assays are all commercially available and therefore can 138 be applied readily to detect SARS-CoV-2 in both human and animal FFPE tissues.
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