Author: Arunkumar Arumugam; Season S Wong
Title: The Potential Use of Unprocessed Sample for RT-qPCR Detection of COVID-19 without an RNA Extraction Step Document date: 2020_4_8
ID: ka3n9pft_18
Snippet: SARS-CoV-2 positive control plasmid (CDC recommended) was obtained from Integrated DNA Technologies (200,000 copies/µL). This was diluted to 10,000 copies/µL in TE buffer and 10 µL of this stock solution was added to 990 µL of VTM to get a final concentration of 100,000 . CC-BY-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/.....
Document: SARS-CoV-2 positive control plasmid (CDC recommended) was obtained from Integrated DNA Technologies (200,000 copies/µL). This was diluted to 10,000 copies/µL in TE buffer and 10 µL of this stock solution was added to 990 µL of VTM to get a final concentration of 100,000 . CC-BY-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.06.028811 doi: bioRxiv preprint copies/mL. A control was prepared by diluting 10 µL of plasmid stock solution in 990 µL of TE buffer. Both the VTM and TE buffer working solutions (4 µL or 400 copies in each reaction) were added into a 20 µL PCR reaction.
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