More than efficacy revealed by single-cell analysis of antiviral therapeutics

Wu Liu; Mehmet U. Caglar; Zhangming Mao; Andrew Woodman; Jamie J. Arnold; Claus O. Wilke; Craig E. Cameron

The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/606715 doi: bioRxiv preprint dependent (Fig. 3d) . A statistically significant difference for mean of the distributions for the 192 remaining parameters was observed at concentrations corresponding to the IC50 and above (Figs. 193 3c, 3e and 3f) . A third signature of antiviral action is therefore revealed with GA. 194 Given the interest in using HSP90 inhibitors as therapeutics for cancer, a variety of 195 compounds exist (Hwang et al., 2009; Sidera and Patsavoudi, 2014) . For example, ganetespib 196 (GS) is an HSP90 inhibitor that differs from GA substantially in chemical structure (compare 197 inset in Fig. S6 to that in Fig. 3a) , has a higher affinity for HSP90 than GA for GA (Fig. S6) . In this case, it is clear that mechanistically identical compounds yield identical 205 signatures at the single-cell level. 206 All rights reserved. No reuse allowed without permission.