The role of post-Golgi transport pathways and sorting motifs in the plasmodesmal targeting of the movement protein (MP) of Ourmia melon virus (OuMV)

Natali Ozber; Paolo Margaria; Charles T. Anderson; Massimo Turina; Cristina Rosa

benthamiana leaves expressing the Golgi marker and GFP:MPwt to further test whether . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/724716 doi: bioRxiv preprint MP sorts in the conventional secretory pathway. In tobacco, BFA inhibits the secretory pathway and causes redistribution of Golgi bodies into ER (reviewed by Robinson et al., 2008; Langhans et al., 2011) . Indeed, treatment with 10 μM BFA caused the Golgi marker to relocate to ER (Fig. 1D , lower panel). While GFP:MP showed a dispersed pattern, similar to that observed with the TGN marker upon BFA treatment (Fig. 1B, lower panel), this pattern did not co-localize with the Golgi marker, suggesting that GFP:MPwt does not enter into the ER-to-Golgi secretory pathway.