Author: Courtney Mycroft-West; Dunhao Su; Stefano Elli; Scott Guimond; Gavin Miller; Jeremy Turnbull; Edwin Yates; Marco Guerrini; David Fernig; Marcelo Lima; Mark Skidmore
Title: The 2019 coronavirus (SARS-CoV-2) surface protein (Spike) S1 Receptor Binding Domain undergoes conformational change upon heparin binding Document date: 2020_3_2
ID: 0d77ojnb_20
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is . https://doi.org/10.1101/2020.02.29.971093 doi: bioRxiv preprint Figure 1 . Interaction of FGF2 and Spike RBD with immobilised heparin. Reducing-end biotinylated heparin was immobilised on a streptavidin functionalized P4SPR sensor surface. PBS running buffer flow rate was 500 µL.min -1 . The data for the three sensing channels are reported as an average response. Protein i.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is . https://doi.org/10.1101/2020.02.29.971093 doi: bioRxiv preprint Figure 1 . Interaction of FGF2 and Spike RBD with immobilised heparin. Reducing-end biotinylated heparin was immobilised on a streptavidin functionalized P4SPR sensor surface. PBS running buffer flow rate was 500 µL.min -1 . The data for the three sensing channels are reported as an average response. Protein injections are delimited by the red arrows. A Injection of 100 nM FGF2. B 64 nM S1 RBD protein. C 64 nM S1 RBD protein over a streptavidin surface (no heparin functionalization). the author/funder. All rights reserved. No reuse allowed without permission.
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