Selected article for: "flow cytometry and surface expression"
Author: Nomani, Masoumeh; Varahram, Mohammad; Tabarsi, Payam; Hashemian, Seyed MohammadReza; Jamaati, Hamidreza; Malekmohammad, Majid; Ghazi, Mona; Adcock, Ian M.; Mortaz, Esmaeil
Title: Decreased neutrophil‐mediated bacterial killing in COVID‐19 patients
  • Cord-id: 5eh9t45r
  • Document date: 2021_6_11
    • ID: 5eh9t45r
    Snippet: The coronavirus disease COVID‐19 was first described in December 2019. The peripheral blood of COVID‐19 patients have increased numbers of neutrophils which are important in controlling the bacterial infections observed in COVID‐19. We sought to evaluate the cytotoxic capacity of neutrophils in COVID‐19 patients. 34 confirmed COVID‐19 patients (29 severe, five mild disease), and nine healthy controls were recruited from the Masih Daneshvari Hospital (Tehran, Iran) from March to May 202
    Document: The coronavirus disease COVID‐19 was first described in December 2019. The peripheral blood of COVID‐19 patients have increased numbers of neutrophils which are important in controlling the bacterial infections observed in COVID‐19. We sought to evaluate the cytotoxic capacity of neutrophils in COVID‐19 patients. 34 confirmed COVID‐19 patients (29 severe, five mild disease), and nine healthy controls were recruited from the Masih Daneshvari Hospital (Tehran, Iran) from March to May 2020. Polymorphonuclear (PMN) cells were isolated from whole blood and incubated with green fluorescent protein (GFP)‐labelled methicillin‐resistant Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA). Bacterial growth was determined by measuring the florescence of co‐cultures of bacteria and neutrophils and reported as the lag time before exponential growth. The number of viable bacteria was determined after 70 hours as colony‐forming units (CFU). The immunophenotype of tested cells was evaluated by flow cytometry. Isolated neutrophils have higher surface expression of CD16 and CD62L with negative markers for PMN‐MDSC. Bacterial growth in the presence of SA (22 ± 0.9 versus 9.2 ± 0.5 h, P < .01) and PA (12.4 ± 0.6 versus 4.5 ± 0.22, P < .01) was significantly reduced in COVID‐19 patients. After 70 h incubation of PMN with bacteria (SA and PA), CFUs were significant increased in COVID‐19 patients SA (2.6 ± 0.09 × 10(8) CFU/mL‐severe patients and 1.4 ± 0.06 × 10(8) CFU/mL‐mild patients, P < .001) and PA (2.2 ± 0.09 × 10(9) CFU/mL‐severe patients and 1.6 ± 0.03 × 10(9) CFU/mL‐mild patients, P < .001). Gentamycin proliferation assays confirmed the presence of intracellular bacteria. Reduced bacterial killing by neutrophils from COVID‐19 patients may be responsible for the high bacterial yield seen in these patients.

    Search related documents:
    Co phrase search for related documents
    • absence presence and lung disease: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
    • absence presence and lung involvement: 1, 2, 3, 4, 5, 6, 7, 8
    • absence presence and luria lb bertani: 1
    • absence presence and lysis buffer: 1, 2, 3, 4, 5
    • lung disease and lysosomal protein: 1