Author: Bishajit Sarkar; Md. Asad Ullah; Fatema Tuz Johora; Masuma Afrin Taniya; Yusha Araf
Title: The Essential Facts of Wuhan Novel Coronavirus Outbreak in China and Epitope-based Vaccine Designing against COVID-19 Document date: 2020_2_11
ID: 7vrm081c_14
Snippet: The online epitope prediction server Immune Epitope Database or IEDB (https://www.iedb.org/) was used for T-cell and B-cell epitope prediction. The database contains a huge collection of experimental data on T-cell epitopes and antibodies [110] . The NetMHCpan EL 4.0 prediction method was used for MHC class-I restricted CD8+ cytotoxic T-lymphocyte (CTL) epitope prediction (for HLA-A*11-01 allele) and the MHC class-II restricted CD4+ helper T-lymp.....
Document: The online epitope prediction server Immune Epitope Database or IEDB (https://www.iedb.org/) was used for T-cell and B-cell epitope prediction. The database contains a huge collection of experimental data on T-cell epitopes and antibodies [110] . The NetMHCpan EL 4.0 prediction method was used for MHC class-I restricted CD8+ cytotoxic T-lymphocyte (CTL) epitope prediction (for HLA-A*11-01 allele) and the MHC class-II restricted CD4+ helper T-lymphocyte (HTL) epitopes were predicted (for HLA DRB1*04-01 allele), using the Sturniolo prediction method [111] . Ten of the top twenty MHC class-I and MHC class-II epitopes were randomly selected based on their antigenicity scores (AS). For, the B-cell lymphocytic epitopes (BCL), with amino acid number of more than ten, were selected for analysis that were predicted using BepiPred linear epitope prediction method [112] [113].
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