The Essential Facts of Wuhan Novel Coronavirus Outbreak in China and Epitope-based Vaccine Designing against COVID-19

Bishajit Sarkar; Md. Asad Ullah; Fatema Tuz Johora; Masuma Afrin Taniya; Yusha Araf

In protein disulfide engineering, disulfide bonds were generated within the 3D structures of the vaccine constructs. In the experiment, the amino acid pairs that had bond energy value less than 2.00 kcal/mol were selected. Since about 90% of the native disulfide bonds in proteins have energy value of less than 2.2 kcal/mol, the bond energy value of 2.00 kcal/mol was selected as the cut-off value for the experiment for better prediction [181] . The CV-1 generated 10 amino acid pairs that had the capability to form disulfide bonds. However, only one pair was selected because they had the bond energy, less than 2.00 kcal/mol: 276 Ser-311 Arg. However, CV-2 and CV-3 generated 04 and 05 pairs of amino acids, respectively, that might form disulfide bonds and no pair of amino acids showed bond energy less than 2.00 Kcal/mol. The selected amino acid pairs of CV-1 formed the mutant version of the original vaccines (Figure 08) . The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.05.935072 doi: bioRxiv preprint