Author: Aran Singanayagam; Joseph Footitt; Benjamin T Kasdorf; Matthias Marczynski; Michael T Cross; Lydia J Finney; Maria-Belen Trujillo Torralbo; Maria Calderazzo; Jie Zhu; Julia Aniscenko; Thomas B Clarke; Philip L Molyneaux; Nathan W Bartlett; Miriam F Moffatt; William O Cookson; Jadwiga Wedzicha; Christopher M Evans; Oliver Lieleg; Patrick Mallia; Sebastian L Johnston
Title: MUC5AC drives COPD exacerbation severity through amplification of virus-induced airway inflammation Document date: 2019_7_22
ID: gg2ctmn7_66
Snippet: Cytokine protein levels in mouse BAL, human sputum supernatants or cell supernatants from in vitro experiments were assayed using commercial "duoset" enzyme-linked immunosorbent assay kits (R&D Systems, Abingdon, UK). The MesoScale Discovery (MSD) platform (Maryland, USA) was used to measure inflammatory mediators IL-1b, IL-6, TNF, CXCL8/IL-8, CXCL10/IP-10 and GM-CSF in sputum supernatants according to manufacturers' instructions as published pre.....
Document: Cytokine protein levels in mouse BAL, human sputum supernatants or cell supernatants from in vitro experiments were assayed using commercial "duoset" enzyme-linked immunosorbent assay kits (R&D Systems, Abingdon, UK). The MesoScale Discovery (MSD) platform (Maryland, USA) was used to measure inflammatory mediators IL-1b, IL-6, TNF, CXCL8/IL-8, CXCL10/IP-10 and GM-CSF in sputum supernatants according to manufacturers' instructions as published previously 15 . Protein levels of human secretory leucocyte proteinase inhibitor (SLPI), elafin, neutrophil elastase, and all mouse BAL proteins were assayed using commercial ELISA assay kits, as previously described 16, 47 . author/funder. All rights reserved. No reuse allowed without permission.
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