Author: K. Reddisiva Prasanth; Minato Hirano; W. Samuel Fagg; Eileen T. McAnarney; Chao Shan; Xuping Xie; Adam Hage; Colette A. Pietzsch; Alexander Bukreyev; Ricardo Rajsbaum; Pei-Yong Shi; Mark T. Bedford; Shelton S. Bradrick; Vineet Menachery; Mariano A. Garcia-Blanco
Title: Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses Document date: 2020_3_27
ID: a0xfb9l4_22
Snippet: To degradate crosslinked proteins and release the precipitated RNA, the input cell-lysate and remained 75% of the beads were treated with Proteinase K (NEB) for 15 min at 37 °C. RNA was The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.24.005900 doi: bioRxiv preprint Scientific) in StepOnePlus Real-Time PCR System (Thermo Fisher Scientific). Primers used in RT-qPCR are shown in Table S1.....
Document: To degradate crosslinked proteins and release the precipitated RNA, the input cell-lysate and remained 75% of the beads were treated with Proteinase K (NEB) for 15 min at 37 °C. RNA was The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.24.005900 doi: bioRxiv preprint Scientific) in StepOnePlus Real-Time PCR System (Thermo Fisher Scientific). Primers used in RT-qPCR are shown in Table S1 . Co-precipitation of the RNA was shown as % Total RNA and Fold Enrichment against DOX (-) condition.
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