Author: K. Reddisiva Prasanth; Minato Hirano; W. Samuel Fagg; Eileen T. McAnarney; Chao Shan; Xuping Xie; Adam Hage; Colette A. Pietzsch; Alexander Bukreyev; Ricardo Rajsbaum; Pei-Yong Shi; Mark T. Bedford; Shelton S. Bradrick; Vineet Menachery; Mariano A. Garcia-Blanco
Title: Topoisomerase III-ß is required for efficient replication of positive-sense RNA viruses Document date: 2020_3_27
ID: a0xfb9l4_22
Snippet: To degradate crosslinked proteins and release the precipitated RNA, the input cell-lysate and remained 75% of the beads were treated with Proteinase K (NEB) for 15 min at 37 °C. RNA was The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.24.005900 doi: bioRxiv preprint Scientific) in StepOnePlus Real-Time PCR System (Thermo Fisher Scientific). Primers used in RT-qPCR are shown in Table S1.....
Document: To degradate crosslinked proteins and release the precipitated RNA, the input cell-lysate and remained 75% of the beads were treated with Proteinase K (NEB) for 15 min at 37 °C. RNA was The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.24.005900 doi: bioRxiv preprint Scientific) in StepOnePlus Real-Time PCR System (Thermo Fisher Scientific). Primers used in RT-qPCR are shown in Table S1 . Co-precipitation of the RNA was shown as % Total RNA and Fold Enrichment against DOX (-) condition.
Search related documents:
Co phrase search for related documents- cell lysate and Real time: 1, 2, 3, 4
- cell lysate and RT qPCR: 1
- cell lysate and Table s1: 1
- enrichment fold and Real time: 1, 2, 3
- enrichment fold and RT qPCR: 1
- Real time and RNA precipitation: 1
- Real time and RT qPCR: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76
- Real time and RT qPCR primer: 1, 2, 3, 4
- Real time and Table s1: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21
- RNA precipitation and RT qPCR: 1, 2, 3, 4, 5, 6, 7
- RT qPCR and Table s1: 1, 2, 3, 4, 5, 6
Co phrase search for related documents, hyperlinks ordered by date