Author: Jessica A. Plante; Kenneth S. Plante; Lisa E. Gralinski; Anne Beall; Martin T. Ferris; Daniel Bottomly; Richard Green; Shannon K. McWeeney; Mark T. Heise; Ralph S. Baric; Vineet D. Menachery
Title: Mucin 4 Protects Female Mice from Coronavirus Pathogenesis Document date: 2020_2_20
ID: 99sbhbuo_38
Snippet: Lyser (Roche, Mannheim, Germany). Debris was pelleted, and lung homogenate was serially 286 diluted 10-fold in PBS. Vero E6 cell monolayers in 6-well plates were infected with 200μl of 287 diluted lung homogenate (10 -1 -10 -6 ) for one hour at 37°C with 5% CO 2 . Monolayers were 288 overlaid with a semi-solid overlay containing MEM supplemented with 2% FBS (HyClone, 289.....
Document: Lyser (Roche, Mannheim, Germany). Debris was pelleted, and lung homogenate was serially 286 diluted 10-fold in PBS. Vero E6 cell monolayers in 6-well plates were infected with 200μl of 287 diluted lung homogenate (10 -1 -10 -6 ) for one hour at 37°C with 5% CO 2 . Monolayers were 288 overlaid with a semi-solid overlay containing MEM supplemented with 2% FBS (HyClone, 289
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