Author: Jessica A. Plante; Kenneth S. Plante; Lisa E. Gralinski; Anne Beall; Martin T. Ferris; Daniel Bottomly; Richard Green; Shannon K. McWeeney; Mark T. Heise; Ralph S. Baric; Vineet D. Menachery
Title: Mucin 4 Protects Female Mice from Coronavirus Pathogenesis Document date: 2020_2_20
ID: 99sbhbuo_46
Snippet: For IHC, sections were deparaffinized with xylene and rehydrated, then placed in near-304 boiling antigen retrieval buffer (10mM Tris base, 1mM EDTA, 0.05% Tween-20, pH 9.0) for 20 305 minutes. Non-specific binding was blocked with 10% normal goat serum (Sigma, St. Louis, MO) 306 and 1% nonfat milk (Lab Scientific, Highlands, NJ) in TBS for two hours at room temperature. 307.....
Document: For IHC, sections were deparaffinized with xylene and rehydrated, then placed in near-304 boiling antigen retrieval buffer (10mM Tris base, 1mM EDTA, 0.05% Tween-20, pH 9.0) for 20 305 minutes. Non-specific binding was blocked with 10% normal goat serum (Sigma, St. Louis, MO) 306 and 1% nonfat milk (Lab Scientific, Highlands, NJ) in TBS for two hours at room temperature. 307
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