Transferrin binding protein B and Transferrin binding protein A 2 expand the transferrin recognition range of Histophilus somni

Anastassia K. Pogoutse; Trevor F. Moraes

While our displacement ELISAs suggested that TbpB is responsible for the binding of H. somni 184 to multiple bovid transferrins, we decided to complement our competitive binding experiments 185 with direct binding assays to gain additional support for this result. We performed direct binding 186 . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/730739 doi: bioRxiv preprint transferrin found in the serum, which ranges between just over 15 µM to just under 90 µM in 209 healthy cattle (see Discussion) (29, 30). We also measured binding of a TbpB from Neisseria 210 meningitidis, a human pathogen, to determine the affinity of TbpBs for non-cognate transferrins. 211