A recombinant infectious bronchitis virus from a chicken with a spike gene closely related to that of a turkey coronavirus

Wang, Yan; Cui, Xuejiao; Chen, Xu; Yang, Shixing; Ling, Yu; Song, Qianben; Zhu, Su; Sun, Luying; Li, Chuang; Li, Yu; Deng, Xutao; Delwart, Eric; Zhang, Wen

Using viral metagenomics, the complete genome sequence of an infectious bronchitis virus (IBV) strain (named ahysx-1) from a fecal sample from a healthy chicken in Anhui province, China, was determined. The genome sequence of ahysx-1 was found to be very similar to that of IBV strain ck/CH/LLN/131040 (KX252787), except for the spike gene region, which is similar to that of a turkey coronavirus strain (EU022526), suggesting that ahysx-1 is a recombinant. Recombination analysis and phylogenetic analysis based on the genomic sequences of ahysx-1 and other related strains confirmed that ahysx-1 appears to be a recombinant resulting from a recombination event that occurred between a chicken coronavirus and a turkey coronavirus. Further studies need to be performed to determine whether this recombinant IBV strain is pathogenic and whether it is transmitted between chickens and turkeys. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s00705-019-04488-3) contains supplementary material, which is available to authorized users.