Selected article for: "correlative light and electron microscopy correlative light"

Author: Jose-Antonio Rodriguez-Rodriguez; Kara L. McKinley; Vitali Sikirzhytski; Jennifer Corona; John Maciejowski; Alexey Khodjakov; Iain M. Cheeseman; Prasad V. Jallepalli
Title: The RZZ complex integrates spindle checkpoint maintenance with dynamic expansion of unattached kinetochores
  • Document date: 2018_4_9
  • ID: 0c4cbwqq_5
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/297580 doi: bioRxiv preprint 5 Mad1-Mad2 and RZZ localize to the fibrous corona, a phosphodependent expansion of the outermost kinetochore layer that persists until end-on microtubule attachment [12, 13] . Kinetochore expansion is thought to accelerate mitotic 'search and capture' by promoting lateral microtubule attachment [10] an.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/297580 doi: bioRxiv preprint 5 Mad1-Mad2 and RZZ localize to the fibrous corona, a phosphodependent expansion of the outermost kinetochore layer that persists until end-on microtubule attachment [12, 13] . Kinetochore expansion is thought to accelerate mitotic 'search and capture' by promoting lateral microtubule attachment [10] and to enhance SAC signaling [11] . RZZ is closely related to endomembrane coatomers that form oligomeric lattices [18, 19] and has been suggested as a "building block" of the corona itself [17, 20] . Consistently two other corona-associated proteins (CENP-E [21] and CENP-F [22] ) also failed to form crescents in KNTC1 -/cells (Fig. 3A) . To obtain definitive evidence, we performed correlative light-electron microscopy in cells that also express CENP-A-GFP as a centromere marker. Serial sectioning revealed circumferential expansion of trilaminar plates and fibrous material in wildtype cells (n=14 kinetochores), whereas the kinetochores of KNTC1 -/cells appeared as compact discs (n=15; Fig. 3B and Fig. S3 ).

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