Cohnella cholangitidis sp. nov., a novel species of the genus Cohnella isolated from a clinical specimen in Korea

Kim, Joon Ki; Choi, Chi-Hwan; Kim, Dae-Won; Kim, Su Yeon; Hwang, Kyu Jam; Lee, Woo-Kon; Shin, Min Kyoung; Jung, Myunghwan; Choi, Young Sill

A Gram-positive, aerobic, rod-shaped bacterium, designated as strain 1605-214(T), was isolated from the blood sample of a patient with cholangitis. Based on its 16S rRNA gene sequence, the strain 1605-214(T) belonged to the genus Cohnella and exhibited 97.9% sequence identity with Cohnella luojiensis DSM 24270(T) (GQ214052). DNA–DNA hybridization, digital DNA–DNA hybridization, and average nucleotide identity values between the two species were 23% ± 1.9, 21.1%, and 77.2%, respectively. The cellular fatty acids of strain 1605-214(T) were mainly comprised of anteiso-C(15:0) (36.1%), iso-C(16:0) (16.5%), and C(16:0) (15.1%). The predominant quinone was menaquinone-7; predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, and aminophospholipid-1. The cell wall peptidoglycan of strain 1605-214(T) contained meso-diaminopimelic acid. DNA G + C content of strain 1605-214(T) was 50.6 mol%. 5187 genes out of a total of 5413 (94.6%) were assigned putative functions using eggNOG v5.0. Based on genotypic characteristics and genomic sequence analysis results, strain 1605-214(T) was confirmed to represent a novel species of genus Cohnella, for which the name Cohnella cholangitidis sp. nov., was proposed. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00203-021-02565-3.