Selected article for: "PCR machine and real time"
Author: Sanchita Bhadra; Miguel A. Saldaña; Hannah Grace Han; Grant L. Hughes; Andrew D. Ellington
Title: Multiplex logic processing isothermal diagnostic assays for an evolving virus
  • Document date: 2018_9_23
    • ID: n5liudlg_46
    Snippet: Real-time measurement of fluorescence accumulation was performed using the LightCycler96 real-time PCR machine (Roche, Basel, Switzerland). Reactions were subjected to isothermal amplification by programming 45 cycles of 150 sec at 65 °C (step 1) followed by 30 sec at 65 °C (step 2). Fluorescence was measured in the FAM channel during step 2 of each amplification cycle. Post-amplification reporter fluorescence was also monitored by programming .....
    Document: Real-time measurement of fluorescence accumulation was performed using the LightCycler96 real-time PCR machine (Roche, Basel, Switzerland). Reactions were subjected to isothermal amplification by programming 45 cycles of 150 sec at 65 °C (step 1) followed by 30 sec at 65 °C (step 2). Fluorescence was measured in the FAM channel during step 2 of each amplification cycle. Post-amplification reporter fluorescence was also monitored by programming the LightCycler96 to hold the assays at 37 ºC for 40 min.

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