Author: MJ Morwitzer; A Corona; L Zinzula; E Fanunza; C Nigri; S Distinto; C Vornholt; V Kumar; E Tramontano; SP Reid
Title: Mutation of Ebola virus VP35 Ser129 uncouples interferon antagonist and replication functions Document date: 2019_8_7
ID: cbyzdnrp_100
Snippet: with 2500 ng of IAV-RNA. After 24 h, total RNA was extracted, reverse transcribed, and 914 subjected to quantitative real-time PCR (RT-qPCR) for the analysis of ISG15 and 2'-5'-915 oligoadenylate synthetase (OAS 2'-5') levels. mRNA expression levels were normalized to the 916 level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Data represent mean ± SD 917 (n=3) of two independent experiments. (C) Effect of VP35-WT and VP35-S129A on ISG56 .....
Document: with 2500 ng of IAV-RNA. After 24 h, total RNA was extracted, reverse transcribed, and 914 subjected to quantitative real-time PCR (RT-qPCR) for the analysis of ISG15 and 2'-5'-915 oligoadenylate synthetase (OAS 2'-5') levels. mRNA expression levels were normalized to the 916 level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Data represent mean ± SD 917 (n=3) of two independent experiments. (C) Effect of VP35-WT and VP35-S129A on ISG56 918 expression upon poly I:C stimulation. HeLa cells were left untransfected or transfected with 919 VP35-WT or VP35-S129A. Twenty-four h after transfection, cells were left untreated or treated 920
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