A Portable Device for LAMP Based Detection of SARS-CoV-2

Sreejith, Kamalalayam Rajan; Umer, Muhammad; Dirr, Larissa; Bailly, Benjamin; Guillon, Patrice; von Itzstein, Mark; Soda, Narshone; Kasetsirikul, Surasak; Shiddiky, Muhammad J. A.; Nguyen, Nam-Trung

This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for the rapid detection of SARS-CoV-2. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 min using clinically relevant starting concentrations of the synthetic template. Time-to-signal data for this device are considerably lower compared to standard quantitative Polymerase Chain Reaction(qPCR) machine (~10–20 min vs. >38 min) for 1 × 10(2) starting template copy number. The device in its fully optimized and characterized state can potentially be used as simple to operate, rapid, sensitive, and inexpensive platform for population screening as well as point-of-need severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) detection and patient management.