Selected article for: "buffer citrate and citrate buffer"
Author: Linlin Zhang; Daizong Lin; Yuri Kusov; Yong Nian; Qingjun Ma; Jiang Wang; Albrecht von Brunn; Pieter Leyssen; Kristina Lanko; Johan Neyts; Adriaan de Wilde; Eric J. Snijder; Hong Liu; Rolf Hilgenfeld
Title: Alpha-ketoamides as broad-spectrum inhibitors of coronavirus and enterovirus replication
  • Document date: 2020_2_10
    • ID: 7n8p9okf_54
    Snippet: Crystals of HCoV-NL63 M pro with 11a were obtained using cocrystallization. The concentrated HCoV-NL63 M pro (45 mg·mL -1 ) was incubated with 5 mM 11a for 4 h at 20℃, followed by setting up crystallization using the vapor diffusion sitting-drop method at 20℃ with equilibration of 1 µL protein (mixed with 1 µL mother liquor) against 500 µL reservoir composed of 0.1 M lithium sulfate monohydrate, 0.1 M sodium citrate tribasic dihydrate, 25.....
    Document: Crystals of HCoV-NL63 M pro with 11a were obtained using cocrystallization. The concentrated HCoV-NL63 M pro (45 mg·mL -1 ) was incubated with 5 mM 11a for 4 h at 20℃, followed by setting up crystallization using the vapor diffusion sitting-drop method at 20℃ with equilibration of 1 µL protein (mixed with 1 µL mother liquor) against 500 µL reservoir composed of 0.1 M lithium sulfate monohydrate, 0.1 M sodium citrate tribasic dihydrate, 25% PEG 1,000, pH 6.0. The crystals were protected by a cryo-buffer containing 0.1 M lithium sulfate monohydrate, 0.1 M sodium citrate tribasic dihydrate, 25% PEG 1,000, 15% glycerol, 2 mM 11a, pH 6.0 and flash-cooled in liquid nitrogen.

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