Author: Richard K. Babbs; Jacob A. Beierle; Julia C. Kelliher; Rose Medeiros; Jeya Anandakumar; Anyaa Shah; Emily J. Yao; Melanie M. Chen; Camron D. Bryant
Title: The demyelinating agent cuprizone induces a male-specific reduction in binge eating in the binge-prone C57BL/6NJ strain Document date: 2019_12_5
ID: 3l8pm0t5_3
Snippet: Blots were then stripped (Thermo Scientific, 46430) at 55°C for 30 min, reblocked with 5% 202 BSA in TBST, incubated in 1:50,000 Beta-actin antibody (Sigma Aldrich, St, Louis, MO, USA, 203 #A2228) in 5% BSA in TBST for 1 h, and then a 1:10,000 dilution of peroxidase conjugated donkey 204 anti mouse antibody for 1 h. Blots were then reimaged for beta actin. Densitometry was conducted 205.....
Document: Blots were then stripped (Thermo Scientific, 46430) at 55°C for 30 min, reblocked with 5% 202 BSA in TBST, incubated in 1:50,000 Beta-actin antibody (Sigma Aldrich, St, Louis, MO, USA, 203 #A2228) in 5% BSA in TBST for 1 h, and then a 1:10,000 dilution of peroxidase conjugated donkey 204 anti mouse antibody for 1 h. Blots were then reimaged for beta actin. Densitometry was conducted 205
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