In vitro evaluation of the activity of terpenes and cannabidiol against Human Coronavirus E229

Chatow, Lior; Nudel, Adi; Nesher, Iris; Hemo, David Hayo; Rozenberg, Perri; Voropaev, Hanna; Winkler, Ilan; Levy, Ronnie; Kerem, Zohar; Yaniv, Zohara; Eyal, Nadav

The activity of a new, terpene-based formulation, code-named NT-VRL-1, against Human Coronavirus (HCoV) strain 229E was evaluated in human lung fibroblasts (MRC-5 cells), with and without the addition of cannabidiol (CBD). The tested formulation exhibited an antiviral effect when it was pre-incubated with the host cells prior to virus infection. The combination of NT-VRL-1 with CBD potentiated the antiviral effect better than the positive controls pyrazofurin and glycyrrhizin. There was a strong correlation between the quantitative results from a cell-viability assay and the cytopathic effect seen under the microscope after 72 h. To the best of our knowledge, this is the first report of activity of a combination of terpenes and CBD against a coronavirus.