Selected article for: "library preparation and native RNA sequencing"
Author: Felix Grünberger; Robert Knüppel; Michael Jüttner; Martin Fenk; Andreas Borst; Robert Reichelt; Winfried Hausner; Jörg Soppa; Sebastien Ferreira-Cerca; Dina Grohmann
Title: Nanopore-based native RNA sequencing provides insights into prokaryotic transcription, operon structures, rRNA maturation and modifications
  • Document date: 2019_12_19
    • ID: e5p4metz_21
    Snippet: We performed whole-genome native RNA sequencing using the Nanopore sequencing technology 321 (referred to as Nanopore native RNA sequencing in this work) that can be applied to any 322 prokaryotic organism. The key steps of the library preparation are shown in Fig. 1a : after 323 enzymatic polyadenylation, the RNA is reverse transcribed to increase sequencing throughput 324 (recommended by ONT) 16 . Subsequently, a motor protein is added that fee.....
    Document: We performed whole-genome native RNA sequencing using the Nanopore sequencing technology 321 (referred to as Nanopore native RNA sequencing in this work) that can be applied to any 322 prokaryotic organism. The key steps of the library preparation are shown in Fig. 1a : after 323 enzymatic polyadenylation, the RNA is reverse transcribed to increase sequencing throughput 324 (recommended by ONT) 16 . Subsequently, a motor protein is added that feeds the RNA into the 325 Nanopore. Following this workflow, native RNAs from prokaryotic organisms can be sequenced. 326

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