Author: Felix Grünberger; Robert Knüppel; Michael Jüttner; Martin Fenk; Andreas Borst; Robert Reichelt; Winfried Hausner; Jörg Soppa; Sebastien Ferreira-Cerca; Dina Grohmann
Title: Nanopore-based native RNA sequencing provides insights into prokaryotic transcription, operon structures, rRNA maturation and modifications Document date: 2019_12_19
ID: e5p4metz_39
Snippet: Additional 16S rRNA processing sites (RNase E: -66, RNase G: 0 5´mature) are also detected ( Fig. 507 4 b,c) . Recent studies were suggesting that the 3´end of the mature 16S rRNA is generated by 3´-5´ 508 ribonucleases activity and/or endonucleolytic cleavage 70,71 . In addition to the mature 16S rRNA 509 3´end, we could observe reads of decreasing length spanning from +33 nt (RNaseIII) to 0 (related 510 to 16S rRNA 3´end), that could a.....
Document: Additional 16S rRNA processing sites (RNase E: -66, RNase G: 0 5´mature) are also detected ( Fig. 507 4 b,c) . Recent studies were suggesting that the 3´end of the mature 16S rRNA is generated by 3´-5´ 508 ribonucleases activity and/or endonucleolytic cleavage 70,71 . In addition to the mature 16S rRNA 509 3´end, we could observe reads of decreasing length spanning from +33 nt (RNaseIII) to 0 (related 510 to 16S rRNA 3´end), that could account for 3´-5´ exonuclease processing activity (or degradation 511 during sample preparation). Together we could accurately identify all the known processing sites 512 at nucleotide resolution in wildtype E. coli. 513 514 Insights into archaeal ribosomal RNA processing 515
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