Author: Felix Grünberger; Robert Knüppel; Michael Jüttner; Martin Fenk; Andreas Borst; Robert Reichelt; Winfried Hausner; Jörg Soppa; Sebastien Ferreira-Cerca; Dina Grohmann
Title: Nanopore-based native RNA sequencing provides insights into prokaryotic transcription, operon structures, rRNA maturation and modifications Document date: 2019_12_19
ID: e5p4metz_4
Snippet: We evaluated the accuracy and reliability of native RNA-seq in comparison to published Illumina-89 based sequencing studying prokaryotic transcriptomes of bacterial (E. coli) and archaeal 90 (Haloferax volcanii, Pyrococcus furiosus) model organisms [26] [27] [28] [29] [30] [31] . The transcriptomic analysis 91 included determination of transcript boundaries, providing, among others, insights into 92 termination mechanisms in archaea. We moreover .....
Document: We evaluated the accuracy and reliability of native RNA-seq in comparison to published Illumina-89 based sequencing studying prokaryotic transcriptomes of bacterial (E. coli) and archaeal 90 (Haloferax volcanii, Pyrococcus furiosus) model organisms [26] [27] [28] [29] [30] [31] . The transcriptomic analysis 91 included determination of transcript boundaries, providing, among others, insights into 92 termination mechanisms in archaea. We moreover demonstrate that the long RNA reads gathered 93 on the ONT platform allow reliable transcriptional unit assignment. Strikingly, we gained insights 94 into the so far poorly understood ribosomal RNA (rRNA) maturation pathway in archaea and 95 detected putative new rRNA processing intermediates. As RNA modifications are retained when 96 sequencing native RNAs, we explore rRNA modifications in prokaryotes. Notably, by expanding 97
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