Author: Minfeng Liao; Yang Liu; Jin Yuan; Yanling Wen; Gang Xu; Juanjuan Zhao; Lin Chen; Jinxiu Li; Xin Wang; Fuxiang Wang; Lei Liu; Shuye Zhang; Zheng Zhang
Title: The landscape of lung bronchoalveolar immune cells in COVID-19 revealed by single-cell RNA sequencing Document date: 2020_2_26
ID: 8l1vfsbc_43
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is . https://doi.org/10.1101/2020.02. 23.20026690 doi: medRxiv preprint operations were performed in BSL-3 laboratory. By passing BALF through a 100 µm nylon cell strainer to filter out lumps and then the supernatant was centrifuged and the cells were re-suspended in the cooled RPMI 1640 complete medium. Then the cells were counted in 0.4% trypan blued, centrifuged and re-suspe.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is . https://doi.org/10.1101/2020.02. 23.20026690 doi: medRxiv preprint operations were performed in BSL-3 laboratory. By passing BALF through a 100 µm nylon cell strainer to filter out lumps and then the supernatant was centrifuged and the cells were re-suspended in the cooled RPMI 1640 complete medium. Then the cells were counted in 0.4% trypan blued, centrifuged and re-suspended at the concentration of 2×10 6 /ml for further use.
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