Author: Campos, Gubio S.; Sardi, Silvia I.; Falcao, Melissa B.; Belitardo, Emilia M.M.A.; Rocha, Danilo J.P.G.; Rolo, Carolina A.; Menezes, Aline D.; Pinheiro, Carina S.; Carvalho, Rejane H.; Almeida, João P.P.; Aguiar, Eric R.G.R.; Pacheco, Luis G.C.
Title: Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19 Cord-id: 65mmlmks Document date: 2020_5_21
ID: 65mmlmks
Snippet: Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus.
Document: Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus.
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