Author: Aravinth Kumar Jayabalan; Diane E. Griffin; Anthony K. L. Leung
Title: Alphavirus nsP3 ADP-ribosylhydrolase Activity Disrupts Stress Granule Formation Document date: 2019_6_20
ID: n8sjpcbs_23
Snippet: CHIKV WT and nsP3 mutants were examined 5.5 h after infection when nsP3 appears as foci in the absence of arsenite (Fig. S4a) . To examine the ability of WT, G32S and Y114A nsP3s to suppress SG formation, infected cells were treated with arsenite for 0.5 h (a time point when ~95% of uninfected cells have developed SGs; data not shown). Consistent with experiments using nsP3 protein expression constructs (Fig. 2a, b) , WT CHIKV infection suppresse.....
Document: CHIKV WT and nsP3 mutants were examined 5.5 h after infection when nsP3 appears as foci in the absence of arsenite (Fig. S4a) . To examine the ability of WT, G32S and Y114A nsP3s to suppress SG formation, infected cells were treated with arsenite for 0.5 h (a time point when ~95% of uninfected cells have developed SGs; data not shown). Consistent with experiments using nsP3 protein expression constructs (Fig. 2a, b) , WT CHIKV infection suppressed SG formation, where only 27% of nsP3-positive cells had canonical (G3BP1/eIF3b double-positive) SGs (Fig. 3a, b) . All rights reserved. No reuse allowed without permission.
Search related documents:
Co phrase search for related documents- CHIKV infection and SG formation: 1
- CHIKV infection and SG formation suppress: 1
- CHIKV infection and WT CHIKV infection: 1, 2, 3, 4, 5
- expression construct and infected cell: 1, 2
- expression construct and protein expression construct: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- foci appear and infected cell: 1
- infected cell and SG formation: 1
- infected cell and time point: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
- infected cell and uninfected cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49
- time point and uninfected cell: 1, 2
Co phrase search for related documents, hyperlinks ordered by date